{"id":6146,"date":"2018-05-21T20:44:19","date_gmt":"2018-05-21T18:44:19","guid":{"rendered":"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/?p=6146"},"modified":"2018-05-21T20:44:19","modified_gmt":"2018-05-21T18:44:19","slug":"fct-aigues-de-barcelona-microbiologia","status":"publish","type":"post","link":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/modul-a\/fct-aigues-de-barcelona-microbiologia\/","title":{"rendered":"FCT Aig\u00fces de Barcelona – Microbiologia"},"content":{"rendered":"

\"\"<\/a><\/p>\n

Si us esteu preguntant que \u00e9s la FCT, a continuaci\u00f3n us ho explicar\u00e9 des de la meva experi\u00e8ncia.<\/p>\n

Per obtenir el t\u00edtol de t\u00e8cnic de Qu\u00edmica Ambiental, es requereix fer 440 hores de formaci\u00f3 en centres de treball o FCT (si no tens cap convalidaci\u00f3). En el meu cas, jo estic realitzant aquesta part al departament de microbiologia del laboratori de Collblanc a l\u2019empresa Aig\u00fces de Barcelona.<\/p>\n

A les meves \u201cpr\u00e0ctiques\u201d, realitzo diferents tasques per poder fer an\u00e0lisis microbiol\u00f2gics de mostres d\u2019aigua, com de la ETAP, de la EDAR, de fonts hornamentals\u2026 Jo m\u2019encarrego de fer controls d\u2019esterilitat de medis de cultius, preparaci\u00f3 de reactius i esterilitzaci\u00f3, per\u00f2 principalment preparo tots els medis de cultius necessaris.<\/p>\n

<\/p>\n

Perqu\u00e8 entengueu el que vull dir amb aix\u00f2, us explicar\u00e9 detalladament com \u00e9s la preparaci\u00f3 d\u2019un medi de cultiu, el TSC-MUP. Aquest medi serveix per la determinaci\u00f3 de Clostridis Sulfit-Reductors, i com estudiareu m\u00e9s endavant, aquests bacteris han de cr\u00e8ixer en anaerobiosi i per tant el medi haur\u00e0 d\u2019estar en aquestes condicions.<\/p>\n

Primer de tot, pessem en balan\u00e7a granat\u00e0ria la quantitat de medi dissecat que requereixi, en aquest cas 23,0g. A continuaci\u00f3 amb probeta mesurem 500mL d\u2019aigua ultrapura. Fem un transv\u00e0s a un matr\u00e0s Erlenmeyer de 1L, primer afegim una mica de aigua, despres l\u2019agar dissecat i finalment l\u2019aigua restant.<\/p>\n

\"\"<\/a><\/p>\n

Un cop preparadala barreja afegim una mosca i posem al bany Maria (dins d\u2019una olla amb aigua i subjectat amb una pesa), aquest pas \u00e9s important per evitar que el medi arribi a edullici\u00f3 i sobresurti del matr\u00e0s amb \u201cefecte volc\u00e0\u201d. Fiquem tot el conjunt a una placa calefactora amb agitador magn\u00e8tic.<\/p>\n

\"\"\u00a0\u00a0<\/a>\"\"<\/a><\/p>\n

Als 15 minuts, el medi estar\u00e0 transparent, i aix\u00ed sabrem que ja el podem treure del bany. El seg\u00fcent pas ser\u00e1 l\u2019esterilitzaci\u00f3, per a aix\u00f2 ho taparem amb paper de plata i celo especial i ho ficarem dins d\u2019una autoclau. Programem 121\u00baC 15 minuts i esperem a que es realitzi. Quan l\u2019autoclau ja ha finalitzat i est\u00e0 a una temperatura per sota dels 95\u00baC, podem treure el matr\u00e0s i refredar-lo a un bany termorregulable a 50\u00baC per tal de que l\u2019agar no arribi a solidificar.<\/p>\n

\u00a0\u00a0<\/a>\"\"<\/a>\u00a0<\/a>\"\"<\/a>\u00a0\"\"<\/p>\n

Mentrestant preparem plaques petites dins d’una campa est\u00e8ril. Aquest medi requereix un reactiu comercial selectiu per clostridis sulfit-reductors, per la seva preparaci\u00f3, dins de la campana, afegim 3mL d’aigua est\u00e8ril al reactiu, tapem i agitem b\u00e9.<\/p>\n

\"\"<\/a>\u00a0 \"\"<\/a>\u00a0\"\"<\/a><\/p>\n

Un cop refredat l\u2019agar, el portem dins la campana i afegim el reactiu, agitem b\u00e9 i ja el podrem distribuir en les plaques. Deixem refredar les plaques i guardem en anaerobiosi. Per aconseguir aix\u00f2, hem fet servir unes caixes que tanquen al buit, i dins disposem una bossa que fa l\u2019anaerobiosi. Un cop distribuides les plaques en caixes, nom\u00e9s caldr\u00e0 etiquetar-les i guardar-les a la nevera, d\u2019aquesta manera es conserven 7 dies.<\/p>\n

\"\"\u00a0<\/a>\"\"<\/a><\/p>\n

Per asegurar-nos que est\u00e0 correctament preparat, caldr\u00e0 dexar una placa 48 hores a 36\u00baC en anaerobiosi i comprobar que no hi creix cap col\u00f2nia. Tamb\u00e9 cal mirar el pH i que estigui entre 7,2 i 7,6.<\/p>\n

\"\"<\/a><\/p>\n

Aqu\u00ed finalitzaria la preparaci\u00f3. Existeixen diferents maneres de preparar un medi, pot estar en tubs o plaques grans o petites, amb certa inclinaci\u00f3\u2026 En el meu cas, \u00e9s preferible preparar de 2\u00a0 a 6 medis cada dia per reposar els gastats per la resta d\u2019analistes del departament i els medis caducats.<\/p>\n

Moltes gr\u00e0cies per dedicar uns minutets a llegar la meva aportaci, espero haver aportat el meu granet de sorra i que us sigui d\u2019ajuda. Molta sort!<\/p>\n","protected":false},"excerpt":{"rendered":"

Si us esteu preguntant que \u00e9s la FCT, a continuaci\u00f3n us ho explicar\u00e9 des de la meva experi\u00e8ncia. Per obtenir […]<\/p>\n","protected":false},"author":209,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[268,173],"tags":[],"class_list":["post-6146","post","type-post","status-publish","format-standard","hentry","category-fct","category-modul-a"],"post_mailing_queue_ids":[],"_links":{"self":[{"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/posts\/6146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/users\/209"}],"replies":[{"embeddable":true,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/comments?post=6146"}],"version-history":[{"count":3,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/posts\/6146\/revisions"}],"predecessor-version":[{"id":6190,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/posts\/6146\/revisions\/6190"}],"wp:attachment":[{"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/media?parent=6146"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/categories?post=6146"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-json\/wp\/v2\/tags?post=6146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}