{"id":2698,"date":"2016-05-16T17:54:58","date_gmt":"2016-05-16T16:54:58","guid":{"rendered":"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/?p=2698"},"modified":"2016-05-17T18:10:45","modified_gmt":"2016-05-17T17:10:45","slug":"filtracio-per-rampa-al-laboratori-de-practiques-de-la-fct","status":"publish","type":"post","link":"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/modul-b\/filtracio-per-rampa-al-laboratori-de-practiques-de-la-fct\/","title":{"rendered":"Filtraci\u00f3 per rampa al laboratori de pr\u00e0ctiques de la FCT"},"content":{"rendered":"<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-2711 size-large\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041-1024x768.jpg\" alt=\"IMG-20160426-WA0004[1]\" width=\"1024\" height=\"768\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041-1024x768.jpg 1024w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041-300x225.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041-200x150.jpg 200w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00041.jpg 1600w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/p>\n<p>Al laboratori de practiques, es fa un control dels aliments, aig\u00fces i compostos farmac\u00e8utics d\u2019empreses productores per determinar-ne la seva qualitat f\u00edsico-qu\u00edmica i microbiol\u00f2gica. Per aquest motiu, s\u2019ha de realitzar els procediments de manera molt controlada a m\u00e9s de etiquetar tots els medis i mostres, ja que n\u2019hi ha de moltes tipologies i diferents par\u00e0metres a analitzar. Tota organitzaci\u00f3 que subministri productes als \u00e9ssers humans han de tenir un servei de control per mesurar-ne la qualitat.<\/p>\n<p><!--more--><\/p>\n<p>Tinc diversos procediments per a realitzar, tals com preparaci\u00f3 de medis, participaci\u00f3 en els m\u00e8todes, an\u00e0lisi f\u00edsico-qu\u00edmic d\u2019aig\u00fces, sembra de microorganismes&#8230; S\u2019ha de destacar que \u00e9s un organisme que et d\u00f3na una responsabilitat i confian\u00e7a, per tal de que aprenguis els coneixements que en un futur et poden ajudar en el m\u00f3n laboral, per tant els has de retornar aquesta confian\u00e7a amb actituds al servei realitzat.<\/p>\n<p><span style=\"text-decoration: underline;\"><strong>T\u00e8cnica de filtraci\u00f3 en rampa d\u2019aig\u00fces:<\/strong><\/span> M\u2019he inclinat per aquesta t\u00e8cnica perqu\u00e8 en l\u2019an\u00e0lisi microbiol\u00f2gic la majoria dels m\u00e8todes es desenvolupen manualment, aquest \u00e9s el que implica m\u00e9s instruments automatitzats.<\/p>\n<ol>\n<li>Preparaci\u00f3 del<span style=\"text-decoration: underline;\"><strong> <a href=\"https:\/\/es.wikipedia.org\/wiki\/Medio_de_cultivo\" target=\"_blank\">medi de cultiu<\/a><\/strong><\/span> espec\u00edfic per el microorganisme que volem identificar o avaluar i posar-lo a un <strong><u><a href=\"https:\/\/es.wikipedia.org\/wiki\/Autoclave\" target=\"_blank\">autoclau<\/a><\/u><\/strong> (procediment semblant a una olla a pressi\u00f3 pel qual s\u2019arriba a temperatures altes i humitat per tal de eliminar els microorganismes i es trobi en unes condicions est\u00e8rils).<\/li>\n<li>Un cop esterilitzat ( 19\u2019 a 121\u00baC ), s\u2019ha de temperar en un bany maria per tal que no es solidifiqui, baixes temperatures, o mati els bacteris de la mostra, altes temperatures al sortir de l\u2019autoclau. Es dispensen els medis en <strong><u><a href=\"https:\/\/ca.wikipedia.org\/wiki\/Placa_de_Petri\" target=\"_blank\">plaques de Petri<\/a><\/u><\/strong>, es retolen i es refreden fins a solidificar.<\/li>\n<li>Un cop preparat el medi es procedeix al filtrat en rampa. Primer s\u2019ha d\u2019homogene\u00eftzar la mostra agitant el flasc\u00f3. Es col\u00b7loca un filtre especial quadriculat sobre el suport de l\u2019instrument, i a sobre l\u2019embut de filtraci\u00f3 (est\u00e8ril). La mostra es diposita a l\u2019embut de filtraci\u00f3 endollat a la rampa. L\u2019instrument es tracta d\u2019un aparell perfectament segellat que crea el buit per tal que l\u2019aigua es filtri en poc temps, utilitzant una <span style=\"text-decoration: underline;\"><strong><a href=\"https:\/\/ca.wikipedia.org\/wiki\/Bomba_(enginyeria)\" target=\"_blank\">bomba<\/a><\/strong><\/span> per a succionar.<\/li>\n<li>Un cop s\u2019acabi el filtrat, amb un volum m\u00ednim de 100 mL ja que sin\u00f3 no es distribuirien els microorganismes uniformement, s\u2019agafa el filtre humit amb unes pinces est\u00e8rils i es diposita en la placa de Petri amb el medi espec\u00edfic que sigui necessari.<\/li>\n<li>Un cop dipositat sobre la placa de Petri, s\u2019incuba a la estufa a una temperatura espec\u00edfica per cada bacteri ( 37\u00baC, 42\u00baC o 30\u00baC) durant un temps determinat.<\/li>\n<li>Passat el per\u00edode previst es fa el comptatge de les col\u00f2nies a la placa i es calcula el nombre de bacteris que hi ha per cada 100mL.<\/li>\n<\/ol>\n<p>En l\u2019an\u00e0lisi microbiol\u00f2gic \u00e9s fonamental treballar en condicions est\u00e8rils, el que implica l\u2019\u00fas de un <span style=\"text-decoration: underline;\"><strong><a href=\"https:\/\/ca.wikipedia.org\/wiki\/Bec_Bunsen\" target=\"_blank\">encenedor Bunsen<\/a><\/strong><\/span> o una <span style=\"text-decoration: underline;\"><strong><a href=\"https:\/\/www.logismarket.es\/erlab\/vitrina-esteril-de-flujo-laminar-vertical\/1342801334-1270817220-p.html\" target=\"_blank\">vitrina est\u00e8ril<\/a><\/strong><\/span>, ja que en l\u2019aire i en tots els objectes hi ha molts microorganismes que poden contaminar la mostra.<\/p>\n<p>Com a conclusi\u00f3 d\u2019aquest any de Cicle de Grau Superior de Qu\u00edmica Ambiental puc dir que no \u00e9s un joc de nens, tot el contrari, implica una organitzaci\u00f3, responsabilitat i aplicaci\u00f3 alta. D\u2019altra banda \u00e9s molt \u00fatil al ser de 1 any i ajuda a accedir a graus universitaris amb una nota de tall alta. Considero que ha sigut un any molt interessant que et d\u00f3na una base de coneixements aplicables a graus cient\u00edfics i una base laboral per tal que en un futur tinguis una oportunitat de feina que un altre no tindria.<\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2713 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011-300x225.jpg\" alt=\"IMG-20160426-WA0001[1]\" width=\"300\" height=\"225\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011-300x225.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011-1024x768.jpg 1024w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011-200x150.jpg 200w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00011.jpg 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2714 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021-300x225.jpg\" alt=\"IMG-20160426-WA0002[1]\" width=\"300\" height=\"225\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021-300x225.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021-1024x768.jpg 1024w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021-200x150.jpg 200w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00021.jpg 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2715 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031-300x225.jpg\" alt=\"IMG-20160426-WA0003[1]\" width=\"300\" height=\"225\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031-300x225.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031-1024x768.jpg 1024w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031-200x150.jpg 200w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00031.jpg 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2716 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051-300x225.jpg\" alt=\"IMG-20160426-WA0005[1]\" width=\"300\" height=\"225\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051-300x225.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051-1024x768.jpg 1024w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051-200x150.jpg 200w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/IMG-20160426-WA00051.jpg 1600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160322_1048411.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2719 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160322_1048411-300x180.jpg\" alt=\"20160322_104841[1]\" width=\"300\" height=\"180\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160322_1048411-300x180.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160322_1048411-1024x614.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><a href=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160323_1159591.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-2720 size-medium aligncenter\" src=\"http:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160323_1159591-300x180.jpg\" alt=\"20160323_115959[1]\" width=\"300\" height=\"180\" srcset=\"https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160323_1159591-300x180.jpg 300w, https:\/\/agora.xtec.cat\/ies-merce-rodoreda\/wp-content\/uploads\/usu644\/2016\/05\/20160323_1159591-1024x614.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<div class=\"ast-oembed-container \" style=\"height: 100%;\"><iframe loading=\"lazy\" title=\"Filtraci\u00f3n de membrana\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/bOzUVmWu95c?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Al laboratori de practiques, es fa un control dels aliments, aig\u00fces i compostos farmac\u00e8utics d\u2019empreses productores per determinar-ne la seva [&hellip;]<\/p>\n","protected":false},"author":43,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center 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